The cytology of Aspergillus nidulans
نویسندگان
چکیده
منابع مشابه
Aspergillus nidulans
Light-dependent conidiation in the filamentous ascomycete, Aspergillus nidulans, is contingent on the allelic state of the velvet (veA) gene. Light dependence is abolished by a mutation in this gene (veAl) , which allows conidiation to occur in the absence of light. We have isolated and characterized six extragenic suppressors of veAl that restore the light-dependent conidiation phenotype. Alle...
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Aspergillus nidulans strain G191 was transformed to hygromycin resistance using plasmid pDH25, which contains the bacterial hygromycin B phosphotransferase gene (hph) fused to promoter elements of the A. nidulans trpC gene. Southern hybridizations of transformants revealed multiple, integrated copies of the vector. A pleiotropic effect conferring increased hygromycin B sensitivity was found to ...
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Mitosis in growing hyphae of haploid and diploid Aspergillus nidulans has been studied with the light microscope in Helly-fixed preparations. At the periphery of the nucleus a sharply defined granule is invariably found which has strong affinity for acid fuchsin. Mitosis begins with the duplication of this granule and the development inside the nucleus of a gradually lengthening fibre between t...
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An electrophoretic karyotype of Aspergillus nidulans has been obtained using contour-clamped homogeneous electric field gel electrophoresis. Six chromosomal bands were separated, with two of the bands migrating as doublets. Using the Schizosaccharomyces pombe and Saccharomyces cerevisiae chromosomes as size standards, we estimate the sizes of the chromosomes to be between 2.9 and 5.0 megabase p...
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DNA replication is precisely coordinated with other cell cycle events to occur once per cell cycle (reviewed by Nasmyth, 1996). The cell achieves strict control over S phase by mobilizing a complex of proteins which activate DNA synthesis at discrete chromosomal origins of replication. A six-protein origin recognition complex (ORC) binds to origins constitutively and serves as an assembly site ...
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ژورنال
عنوان ژورنال: Genetical Research
سال: 1960
ISSN: 0016-6723,1469-5073
DOI: 10.1017/s0016672300000434